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Análise da capacidade antissética de esponjas de celulose oxidada em digluconato de clorexidina 0,2% v/v: estudo piloto
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Abstract(s)
As extrações dentárias são procedimentos efetuados rotineiramente pelos Médicos Den-tistas e que na grande maioria dos casos não produz mais que um incómodo passageiro de rápida cicatrização. No entanto, não é rara a ocorrência de complicações relacionadas com as extrações dentárias. As complicações pós-operatórias podem ser locais ou sisté-micas. A complicação infecciosa local mais frequente é a alveolite. Existem vários tipos de alveolite, sendo a alveolite seca a mais comum.
O objetivo deste estudo piloto in vitro foi avaliar a capacidade antissética de 3 esponjas hemostáticas reabsorvíveis de celulose oxidada de marcas comerciais distintas: hemos-pon®, spongostan® e gelatamp®, impregnadas em digluconato de clorexidina a 0,2% v/v.
Foi recolhida saliva de 6 voluntários, com idades compreendidas entre os 23 e os 30 anos, com evidência de saúde oral (OMS), sem história terapêutica antibiótica ou medicamen-tosa, sem patologia periodontal ativa ou exodontias. As esponjas foram impregnadas com uma solução de 0,2%v/v de digluconato de clorexidina e colocadas no respetivo tubo com 5 ml de saliva. A amostragem foi realizada às 0, 24, 48, 72 e 96 horas. As amostras foram submetidas ao método das diluíções decimais sucessivas e realizado o respectivo plaque-amento em gelose de sangue. Após o período de incubação, os resultados foram visuali-zados e expressos em Unidades Formadoras de Colónias /mL.
Dentro das limitações do desenho do estudo, verificou-se que a solução de digluconato de clorexidina a 0.2% v/v apresentou capacidade antissética bactericida e verificou-se uma desintegração mais lenta das esponjas hemostáticas.
Dental extractions are procedures routinely performed by dentists and in the vast majority of cases it produces no more than a transient discomfort of rapid healing. However, there is no rare occurrence of complications related to tooth extractions. Post-operative com-plications may be local or systemic. The most frequent local infectious complication is alveolitis. There are several types of alveolitis, and the most common is dry alveolitis. The objective of this in vitro pilot study was to evaluate the antiseptic capacity of 3 reab-sorbable hemostatic sponges of oxidised cellulose from distinct trademarks Hemospon®, Spongostan® and Gelatamp®, impregnated in chlorhexidine digluconate of 0.2% v/v. Saliva was collected from 6 volunteers, aged between 23 and 30 years, with evidence of oral health (WHO), with no antibiotic or drug therapeutic history, without active perio-dontal pathology or exodontias. The sponges were impregnated with a solution of 0.2%v/v and placed in the respective tube with 5 ml of saliva. The sampling was per-formed at 0, 24, 48, 72 and 96 hours. The samples were submitted to the method of suc-cessive decimal dilutions and the respective plating was performed in blood agar. After the incubation period, the results were visualized and expressed in Colony Forming Units/mL. Within the limitations of the study design, it was found that chlorhexidine digluconate solution at 0.2% v/v presented bactericidal antissetic capacity and there was a slower disintegration of hemostatic sponges.
Dental extractions are procedures routinely performed by dentists and in the vast majority of cases it produces no more than a transient discomfort of rapid healing. However, there is no rare occurrence of complications related to tooth extractions. Post-operative com-plications may be local or systemic. The most frequent local infectious complication is alveolitis. There are several types of alveolitis, and the most common is dry alveolitis. The objective of this in vitro pilot study was to evaluate the antiseptic capacity of 3 reab-sorbable hemostatic sponges of oxidised cellulose from distinct trademarks Hemospon®, Spongostan® and Gelatamp®, impregnated in chlorhexidine digluconate of 0.2% v/v. Saliva was collected from 6 volunteers, aged between 23 and 30 years, with evidence of oral health (WHO), with no antibiotic or drug therapeutic history, without active perio-dontal pathology or exodontias. The sponges were impregnated with a solution of 0.2%v/v and placed in the respective tube with 5 ml of saliva. The sampling was per-formed at 0, 24, 48, 72 and 96 hours. The samples were submitted to the method of suc-cessive decimal dilutions and the respective plating was performed in blood agar. After the incubation period, the results were visualized and expressed in Colony Forming Units/mL. Within the limitations of the study design, it was found that chlorhexidine digluconate solution at 0.2% v/v presented bactericidal antissetic capacity and there was a slower disintegration of hemostatic sponges.
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Keywords
Alveolite Clorexidina Esponja hemostática Microbioma oral Alveolar osteite Chlorohexidine Hemostatic sponges Oral microbiome