Repository logo
 
Publication

Effect of 1-carbaldehyde-3,4-dimethoxyxanthone on prostate and HPV-18 positive cervical cancer cell lines and on human THP-1 macrophages

2021Journal article Restricted access
Simple item page
dc.contributor.authorMedeiros, R.
dc.contributor.authorHorta, Bruno
dc.contributor.authorFreitas-Silva, Joana
dc.contributor.authorSilva, Jani
dc.contributor.authorDias, Francisca
dc.contributor.authorSousa, Emília
dc.contributor.authorPinto, Madalena
dc.contributor.authorCerqueira, Fátima
dc.date.accessioned2021-07-13T11:42:19Z
dc.date.available2021-07-13T11:42:19Z
dc.date.issued2021
dc.description.abstractXanthone derivatives have shown promising antitumor properties, and 1-carbaldehyde-3,4- dimethoxyxanthone (1) has recently emerged as a potent tumor cell growth inhibitor. In this study, its effect was evaluated (MTT viability assay) against a new panel of cancer cells, namely cervical cancer (HeLa), androgen-sensitive (LNCaP) and androgen-independent (PC-3) prostate cancer, and nonsolid tumor derived cancer (Jurkat) cell lines. The effect of xanthone 1 on macrophage functions was also evaluated. The effect of xanthone 1-conditioned THP-1 human macrophage supernatants on the metabolic viability of cervical and prostate cancer cell lines was determined along with its interference with cytokine expression characteristic of M1 profile (IL-1 ≤ β; TNF-α) or M2 profile (IL-10; TGF-β) (PCR and ELISA). Nitric oxide (NO) production by murine RAW264.7 macrophages was quantified by Griess reaction. Xanthone 1 (20 µM) strongly inhibited the metabolic activity of the cell lines and was significantly more active against prostate cell lines compared to HeLa (p < 0.05). Jurkat was the cell most sensitive to the effect of xanthone 1. Compound 1-conditioned IL-4-stimulated THP-1 macrophage supernatants significantly (p < 0.05) inhibited the metabolic activity of HeLa, LNCaP, and PC-3. Xanthone 1 did not significantly affect the expression of cytokines by THP-1 macrophages. The inhibiting effect of compound 1 observed on the production of NO by RAW 264.7 macrophages was moderate. In conclusion, 1-carbaldehyde-3,4-dimethoxyxanthone (1) decreases the metabolic activity of cancer cells and seems to be able to modulate macrophage functions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/molecules26123721pt_PT
dc.identifier.eissn1420-3049
dc.identifier.urihttp://hdl.handle.net/10284/10073
dc.language.isoengpt_PT
dc.publisherMDPIpt_PT
dc.subject1-carbaldehyde-3,4-dimethoxyxanthonept_PT
dc.subjectAntitumorpt_PT
dc.subjectProstate cancerpt_PT
dc.subjectCervical cancerpt_PT
dc.subjectImmunomodulationpt_PT
dc.subjectMacrophage functionpt_PT
dc.subjectCytokinespt_PT
dc.titleEffect of 1-carbaldehyde-3,4-dimethoxyxanthone on prostate and HPV-18 positive cervical cancer cell lines and on human THP-1 macrophagespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12pt_PT
oaire.citation.startPage3721pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume26pt_PT
person.familyNameMedeiros
person.familyNameCerqueira
person.givenNameRui
person.givenNameFátima
person.identifier37604
person.identifier812695
person.identifier.ciencia-idC51F-5DBE-9C51
person.identifier.ciencia-idCF14-52ED-5DA0
person.identifier.orcid0000-0003-3010-8373
person.identifier.orcid0000-0003-4513-4654
person.identifier.ridC-7938-2009
person.identifier.ridM-7557-2013
person.identifier.scopus-author-id7006241641
person.identifier.scopus-author-id6603346911
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5ffb7965-d9af-4ba1-a81b-b0aed28de206
relation.isAuthorOfPublication2705e35c-97f6-4de2-bbf1-d680342760e9
relation.isAuthorOfPublication.latestForDiscovery2705e35c-97f6-4de2-bbf1-d680342760e9

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Document_06_2021.pdf
Size:
1.35 MB
Format:
Adobe Portable Document Format
Download

Collections

FCS - Artigos em Revistas Científicas Internacionais com Arbitragem Científica