Percorrer por autor "Moura Teles, Ana"
A mostrar 1 - 7 de 7
Resultados por página
Opções de ordenação
- Bacterial contamination of Gutta-Percha points from different brands and the efficacy of a chairside disinfection protocolPublication . Bracciale, Francesca; Marino, Nicole; Noronha, Anariely; Manso, Maria da Conceição; Gavinha, Sandra; Lopes Cardoso, Inês; Pina, Cristina; Moura Teles, AnaObjective: To evaluate the bacterial contamination of different brands of Gutta-Percha (GP) points routinely used in clinical practice and the efficacy of a chairside disinfection protocol with sodium hypochlorite. Methods: GP points (n=240), in sizes A, B, C, D, K15, K20, K25, K30, K35, K40, F1, F2, F3 (Dentsply®, Proclinic®, ProTaper® and R&S®), were randomly sampled from commercial packages already in use. These were added directly to Fluid Thioglycolate Medium (one GP point per tube) and incubated at 37ºC for 21 days. During this period, the presence/absence of turbidity was evaluated. To evaluate the efficacy of a chairside disinfection protocol, all detected contaminated GP points were immersed for 1 minute in 10 mL of 5.25% sodium hypochlorite, followed by 5 minutes in 10 mL of detergent solution (3% Tween 80 and 5% sodium thiosulfate) and a final rinse with 10 mL of sterile distilled water and incubated. The data was analysed using the chi-square test and differences between characteristics of dichotomic variables were performed using the binomial test. The significance level was set at P<0.05. Results: Bacterial growth was observed in 22.9% of the total study samples. Dentsply® and R&S® showed the highest level of contamination, 47.3% each, although without significant differences to the other commercial brands. The most contaminated GP point size was K30 (16.4%). The chairside disinfection protocol was effective in disinfection of 76.4% of GP points (P<0.001). Conclusion: A real small number of GP points in clinical use harboured bacteria, including after the Chairside Disinfection Protocol that, anyway, proved to be effective. No significant difference was observed between tested commercial brands.
- Degree of contamination of Gutta-Percha points by Staphylococcus aureus (MRSA/MSSA) strainsPublication . Moura Teles, Ana; Pina, Cristina; Lopes Cardoso, Inês; Tramontana, Antea; Cardoso, Miguel; Duarte, Ana Sofia; Bartolomeu, Maria; Noites, RitaMethicillin-resistant Staphylococcus aureus (MRSA) is considered one of the most harmful bacteria to human health. Dentistry, like all healthcare disciplines, places great emphasis on preventing scenarios that may result in cross-infection. Although various tested and already used materials are suitable for filling the root canal system, Gutta-Percha (GP) remains the preferred and widely accepted gold standard. Objective: We performed an in vitro analysis of the contamination of GP points, regarding the strains of Methicillin-resistant (MRSA) and Methicillin-sensitive (MSSA) Staphylococcus aureus, using classical microbiology methods and molecular biology techniques. Methods: Gutta-Percha points of two different brands from opened packages (already in use for 1 month) were collected for analysis. The assessment involved incubating the GP points in Brain Heart Infusion (BHI) medium to detect microbial growth. Growing microorganisms were plated on a selective and differential chromogenic medium for MRSA/MSSA strains, and the identification of isolates was confirmed by Polymerase Chain Reaction (PCR). In the case of microbial growth, the GP point was submitted to a disinfection protocol. Results: From the 315 collected GP points, only 6 (1.9%) resulted in being positive for microbial growth. After confirmation by PCR, only one sample of the six GP points was contaminated by MRSA, and the remaining five were MSSA-contaminated. The disinfection protocol was effective in all contaminated GP points. Conclusions: The Gutta-Percha points from opened pre-sterilized packages showed a very low degree of contamination by MRSA/MSSA. However, the detection of MSSA and MRSA strains raises concerns about potential contamination in dental clinic environments, and this risk cannot be considered negligible.
- Genetic bases in the reabsorption of the mandibular residual crestPublication . Moura Teles, Ana; Colombo, Juan; Lopes Cardoso, InêsThe increase in life span of the human being leads to the appearance of new challenges for the health sciences. As a result of getting old, chronic and degenerative diseases started to occupy a predominant role in clinical research in the last decades. It is the case of degenerative processes that occur in the oral cavity after teeth loss.Mandibular residual ridge resorption is a widely studied process from the middle of last century and muchresearch relate this process with multiple factors. Recently, the understanding of the biological processes that occur after tooth extraction has been amplified.In this work, genetic factors involved in mandibular residual ridge resorption are discussed and the relationship between single nucleotide polymorphisms of some genes and mandibular residual ridge resorptionare stablished.
- Genetic involvement in dental treatment fearPublication . Khodabakhshi, Donja; Pina, Cristina; Moura Teles, Ana; Lopes Cardoso, InêsBeing a global health concern, dental fear and anxiety is estimated to affect at least one in ten individuals all around the globe. Ensuing the vicious circle of dental fear comes detrimental outcomes in quality of life. Despite the wide range of knowledge concerning the environmental factors that contribute to the acquisition of dental fear and anxiety, the prevalence has not decreased, giving researchers the possibility to highlight other concomitant etiological factors. The possible hereditary or genetic component is most recently being explored, although facing tremendous dynamical elements to substantially delineate its role. The purpose of this review was to explore, reflect and call attention to the current knowledge of the genetic involvement of dental treatment fear and anxiety. Although findings mostly stress the relation between dental fear to other phenotypes giving rise to individual vulnerability, there are studies disclosing suggestive heritability rates for dental fear and anxiety.
- Hypohidrotic ectodermal dysplasia and its manifestations in the oral cavityPublication . Cardoso, Jennifer; de Faria Carvalho, Célio Domingos; Carvalho Silva, Cátia; Moura Teles, Ana; C. Leal, M. Fernanda; Lopes Cardoso, InêsThe Ectodermal Dysplasias generally present orofacial manifestations, such as skeletal discrepancies and dental alterations. Therefore, the role of a paediatric dentist in the detection and recognition of these repercussions can be crucial in early diagnosis of the disease. The oral rehabilitation of paediatric patients with this condition is extremely important, ideally, at a very early stage, yet contributing for the re-establishment of normal chewing, swallowing and phonetics functions, and, naturally, aesthetics increase. The purpose of this narrative review aims to elucidate dentists about their role in the detection, diagnosis, treatment and monitoring of the Ectodermal Dysplasia’ oral manifestations in paediatric patients, through the presentation of general physical and specific craniofacial characteristics.
- Intracanal medication as antibiotic control in case of permanent mature teeth with necrotic pulp or apical periodontitisPublication . Moura Teles, Ana; Lassarat, Rebecca; Pina, Cristina; Lopes Cardoso, InêsNon-Surgical Endodontic Treatment consists in the removal of dental pulp and the elimination of microorganisms that cause infection of the pulp space. This treatment can be performed in a single or in multiple sessions and, sometimes, the use of Intracanal Medication may be necessary. The purpose of Intracanalar Medication is to reach bacteria present in places inaccessible to instrumentation or irrigants and, also, to inhibit the invasion of the root canal system by bacteria or its products by coronal, lateral or apical communications with the outside through dentinal tubules, apical foramen or accessories root canals. The present work is a narrative review of the Literature that aims to analyse the different opinions among authors, in the case of permanent mature teeth with diagnosis of necrosis with or without apical periodontitis associated, and to understand in which situations it is necessary to apply Intracanal Medication, which should be chosen and how it should be applied. In this way, it is also intended that this work can be a useful guide for clinicians.
- Relevant factors for dental care in planning the response against covid-19: a narrative reviewPublication . Aires, Thadeu Laranja; Lopes Cardoso, Inês; Pina, Cristina; Moura Teles, AnaThe main objective of this work was to analyze, through a review of current references, how different factors correlate to shape the response measures implemented by health authorities to the ongoing pandemic event, in the context of dental care.The COVID-19 pandemic has claimed more than 14million lives directly or indirectly, being a special challenge for society and health care systems. The role of dentists during a pandemic crisis is to provide essential dental care while preventing transmission of the virus. However, this virus has some traits that may increase the risk of cross infection in dental offices, due to aerosols produced during several dental treatments. To address this problem, health authorities have prepared specific protocols to prevent cross-contamination by this virus in dental offices, allowing patients to continue receiving dental care during the pandemic period.