Nascimento, HenriqueVieira, EmíliaCoimbra, SusanaCatarino, CristinaCosta, ElísioBronze-da-Rocha, ElsaRocha-Pereira, PetronilaCarvalho, MárciaMansilha, Helena FerreiraRêgo, CarlaSantos, Rosário dosSantos-Silva, AliceBelo, Luís2021-07-022021-07-022016Nascimento, H., Vieira, E. , Coimbra, S., Catarino, C., Costa, E., Bronze-da-Rocha, E., Rocha-Pereira, P., Carvalho, M., Mansilha, H.F., Rêgo, C., Santos, R., Santos-Silva, A. and Belo, L. (2016). Adipokine gene single-nucleotide polymorphisms in portuguese obese adolescents: associations with plasma concentrations of adiponectin, resistin, IL-6, IL-1β, and TNF-α. Childhood Obesity. pp.300-313. http://doi.org/10.1089/chi.2015.02352153-2168http://hdl.handle.net/10284/10033Background: The genetic contribution to obesity and to circulating adipokine levels has not been completely clarified. We aimed to evaluate adipokine genes' single-nucleotide polymorphism (SNP) prevalence and its association with circulating adipokine levels and risk factors for cardiovascular disease in an obese Portuguese pediatric population. Methods: Two hundred forty-eight obese adolescents (mean age 13.4 years old; 47.2% females) participated in a cohort study. We screened 12 SNPs by direct sequencing in five adipokine genes: adiponectin (ADIPOQ: rs16861194, rs17300539, rs266729, rs2241766, rs1501299), interleukin-1β (IL-1β; rs1143627), IL-6 (IL-6; rs1800795), tumor necrosis factor-α (TNF-α; rs1800629), and resistin (RETN; rs1862513, rs3219177, rs3745367, rs3745368). Biochemical analysis included determination of circulating adipokines, C-reactive protein (CRP) levels, lipid profile, and markers of insulin resistance. Results: Compared to males, females presented higher circulating levels of insulin, adiponectin, IL-6, resistin, and leptin concentrations, but lower TNF-α levels. No statistically significant differences were found for genotype or allelic distributions between genders. In the whole sample population, adiponectin levels were influenced by ADIPOQ rs17300539 (c.-1138G>A; lower in subjects with GG genotype). When only males were considered, IL-1β, IL-6, and TNF-α levels were associated with ADIPOQ rs1501299 (c.214 + 62G>T; higher in GG subjects). TNF-α concentrations were modulated by TNF-αrs1800629 (c.-488G>A; lower in GG males), RETN rs1862513 (c.-216C>G; higher in CC subjects), and RETN rs3219177 (c.118 + 39C>T; higher in CC subjects). Leptin levels were influenced by IL-1β rs1143627 (c.-118C>T) presenting TT individuals' lower levels. Conclusions: Our data demonstrate that in pediatric obese patients, some adipokine gene SNPs have an association with circulating adipokine levels and lipid profile.engAdipokinesAdolescentCohort StudiesFemaleGenetic predisposition to diseaseGenotypeHumansInsulin resistanceInterleukin-1βInterleukin-6MalePediatric obesityPolymorphismPortugalResistinTumor necrosis factor-αSingle nucleotidejournal article10.1089/chi.2015.02352153-2176